细胞名称：人肝癌细胞HepaRG
产品规格：T25培养瓶x1；1.5ml冻存管x2
细胞数量：1x10^6；1x10^6
保存温度：37℃；-198℃
运输方式：常温保温运输；干冰运输
安全等级：1
用途限制：仅供科研2类
培养体系：90%DMEM+10%FBS+1%三抗
培养温度：37℃
二氧化碳浓度：5%
简介：人肝癌细胞HepaRG取自女性供体，贴壁培养。
注释：Registration:International Depositary Authority,Pasteur Institute Collection Nationale de Cultures de Micro-organismes(CNCM);I-2652.
Characteristics:Can be induced to differentiate into hepatocyte-like cells by exposure to DMSO.
Characteristics:Cell line main applications are sugar and lipid metabolism,physiopathology;drug metabolism and toxicology and viral infection.
Omics:Deep proteome analysis.
Omics:Membrane proteome analysis.
Omics:miRNA expression profiling.
Omics:Transcriptome analysis.
Anecdotal:The'RG'in the cell name stands for Rumin and Gripon,the two scientists that were influential in the establishment of this cell line.
基因突变：/
HLA信息：/
STR信息：/
参考文献：PubMed=12432097;DOI=10.1073/pnas.232137699
Gripon P.,Rumin S.,Urban S.,Le Seyec J.,Glaise D.,Cannie I.,Guyomard C.,Lucas J.,Trepo C.,Guguen-Guillouzo C.
Infection of a human hepatoma cell line by hepatitis B virus.
Proc.Natl.Acad.Sci.U.S.A.99:15655-15660(2002)
PubMed=17241619;DOI=10.1016/j.cbi.2006.12.003
Guillouzo A.,Corlu A.,Aninat C.,Glaise D.,Morel F.,Guguen-Guillouzo C.
The human hepatoma HepaRG cells:a highly differentiated model for studies of liver metabolism and toxicity of xenobiotics.
Chem.Biol.Interact.168:66-73(2007)
PubMed=17393521;DOI=10.1002/hep.21536
Cerec V.,Glaise D.,Garnier D.,Morosan S.,Turlin B.,Drenou B.,Gripon P.,Kremsdorf D.,Guguen-Guillouzo C.,Corlu A.
Transdifferentiation of hepatocyte-like cells from the human hepatoma HepaRG cell line through bipotent progenitor.
Hepatology 45:957-967(2007)
Patent=US7456018
Gripon P.,Guguen-Guillouzo C.,Trepo C.,Rumin S.
Human hepatoma lines,methods for obtaining same and uses thereof.
Patent number US7456018,25-Nov-2008
PubMed=20228232;DOI=10.1124/dmd.109.031831
Hart S.N.,Li Y.,Nakamoto K.,Subileau E.-A.,Steen D.,Zhong X.-B.
A comparison of whole genome gene expression profiles of HepaRG cells and HepG2 cells to primary human hepatocytes and human liver tissues.
Drug Metab.Dispos.38:988-994(2010)
PubMed=20645056;DOI=10.1007/978-1-60761-688-7_13
Marion M.-J.,Hantz O.,Durantel D.
The HepaRG cell line:biological properties and relevance as a tool for cell biology,drug metabolism,and virology studies.
Methods Mol.Biol.640:261-272(2010)
PubMed=21414303;DOI=10.1016/j.bcp.2011.03.004
Ceelen L.,De Spiegelaere W.,David M.,De Craene J.,Vinken M.,Vanhaecke T.,Rogiers V.
Critical selection of reliable reference genes for gene expression study in the HepaRG cell line.
Biochem.Pharmacol.81:1255-1261(2011)
PubMed=22568886;DOI=10.1517/17425255.2012.685159
Andersson T.B.,Kanebratt K.P.,Kenna J.G.
The HepaRG cell line:a unique in vitro tool for understanding drug metabolism and toxicology in human.
Expert Opin.Drug Metab.Toxicol.8:909-920(2012)
PubMed=22594799;DOI=10.3109/13813455.2012.683442
Samanez C.H.,Caron S.,Briand O.,Dehondt H.,Duplan I.,Kuipers F.,Hennuyer N.,Clavey V.,Staels B.
The human hepatocyte cell lines IHH and HepaRG:models to study glucose,lipid and lipoprotein metabolism.
Arch.Physiol.Biochem.118:102-111(2012)
PubMed=22643240;DOI=10.1016/j.tiv.2012.05.008
Antherieu S.,Chesne C.,Li R.,Guguen-Guillouzo C.,Guillouzo A.
Optimization of the HepaRG cell model for drug metabolism and toxicity studies.
Toxicol.In Vitro 26:1278-1285(2012)
PubMed=22857383;DOI=10.1186/1477-5956-10-47
Sokolowska I.,Dorobantu C.,Woods A.G.,Macovei A.,Branza-Nichita N.,Darie C.C.
Proteomic analysis of plasma membranes isolated from undifferentiated and differentiated HepaRG cells.
Proteome Sci.10:47-47(2012)
PubMed=23887712;DOI=10.1038/ncomms3218
Nault J.-C.,Mallet M.,Pilati C.,Calderaro J.,Bioulac-Sage P.,Laurent C.,Laurent A.,Cherqui D.,Balabaud C.,Zucman-Rossi J.
High frequency of telomerase reverse-transcriptase promoter somatic mutations in hepatocellular carcinoma and preneoplastic lesions.
Nat.Commun.4:2218-2218(2013)
PubMed=26160117;DOI=10.1093/toxsciv136
Sison-Young R.L.C.,Mitsa D.,Jenkins R.E.,Mottram D.,Alexandre E.,Richert L.,Aerts H.,Weaver R.J.,Jones R.P.,Johann E.,Hewitt P.G.,Ingelman-Sundberg M.,Goldring C.E.P.,Kitteringham N.R.,Park B.K.
Comparative proteomic characterization of 4 human liver-derived single cell culture models reveals significant variation in the capacity for drug disposition,bioactivation,and detoxication.
Toxicol.Sci.147:412-424(2015)
PubMed=26694163;DOI=10.1371/journal.pone.0144924
Janiszewska J.,Szaumkessel M.,Kostrzewska-Poczekaj M.,Bednarek K.,Paczkowska J.,Jackowska J.,Grenman R.,Szyfter K.,Wierzbicka M.,Giefing M.,Jarmuz-Szymczak M.
Global miRNA expression profiling identifies miR-1290 as novel potential oncomiR in laryngeal carcinoma.
PLoS ONE 10:E0144924-E0144924(2015)
PubMed=27169750;DOI=10.1038ep24709
Sharanek A.,Burban A.,Burbank M.,Le Guevel R.,Li R.,Guillouzo A.,Guguen-Guillouzo C.
Rho-kinase/myosin light chain kinase pathway plays a key role in the impairment of bile canaliculi dynamics induced by cholestatic drugs.
Sci.Rep.6:24709-24709(2016)
PubMed=27780834;DOI=10.1124/dmd.116.072603
van der Mark V.A.,de Waart D.R.,Shevchenko V.,Oude Elferink R.P.J.,Chamuleau R.A.F.M.,Hoekstra R.
Stable overexpression of the constitutive androstane receptor reduces the requirement for culture with dimethyl sulfoxide for high drug metabolism in HepaRG cells.
Drug Metab.Dispos.45:56-67(2017)
PubMed=27975304;DOI=10.1007/978-1-4939-6700-1_2
Ni Y.,Urban S.
Hepatitis B virus infection of HepaRG cells,HepaRG-hNTCP cells,and primary human hepatocytes.
Methods Mol.Biol.1540:15-25(2017)
PubMed=28904299;DOI=10.2131/jts.42.641
Tomida T.,Ishimura M.,Iwaki M.
A cell-based assay using HepaRG cells for predicting drug-induced phospholipidosis.
J.Toxicol.Sci.42:641-650(2017)
细胞操作说明书下载：细胞操作说明书
